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Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Tel: 86-21-54336592
Contact Person:
Mr zhao

Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature

Model Number : Re15
MOQ : 50ug
Price : USD142
Payment Terms : T/T
Supply Ability : 10000G
Delivery Time : 4days
Brand Name : YaxinBio
Certification : ISO9001
Place of Origin : China
Packaging Details : 50ug
Activity : ≥10u/mg
purification : HPLC
character : freeze-dried powder
molecular : 67KD
perfect PH : 9.0
nature : double base enzyme
temperature : 2-8℃
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Kex-2 Protease Double Base Enzyme that the perfect PH is 9.0 With 2-8°C Storage Temperature


Kex-2 Protease


PRODUCT INFORMATION
Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids.At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.


MAIN FEATURES

SourcePichia pastoris
AppearanceWhite or off white powder
Specific activity≥10.0 units/mg pro.
Purity(SDS-PAGE)Single major band
Molecular Weight(SDS-PAGE)24.0±2.4 kDa

UNIT DEFINITION: One unit of Kex2 activity will release 1μmol 4-nitroaniline per minute in a reaction volume of 3.0 ml at pH8.0 and 25℃ with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.


RECOMMEND USAGE

Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl,2 mM Ca2 + or HEPES, 5 mM Ca2 +. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2 + buffer to make the final concentration is about 1-10 mg/ml,then make suitable packing as your requirement and store at -20℃.Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2 + or HEPES, 5 mM Ca2 + buffer.

Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.


Product Tags:

chemical ingredients

      

saccharomyces cerevisiae yeast

      
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