Recombinant Aprotinin EC 22.214.171.124 Aprotinin from Bovine Lung 3
Aprotinin is a competitive serine protease inhibitor that inhibits
trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms
stable complexes with and blocks the active sites of enzymes.
Binding is reversible with most aprotinin-protease complexes and
dissociating at pH >10 or <3. Effective concentration is
equimolar with protease.
Anonym: Trypsin Inhibitor
Recombinant Animal Components Free
Recombinant aprotinin is expressed in E. Coli, and purified by HPLC. It contains no animal-derived components.
This is a recombinant form of bovine lung aprotinin, which is
traditionally isolated from bovine lung by methods involving
fractional precipitation, gel filtration, and ion exchange
One trypsin inhibitor unit (EPU) will decrease the activity of 2
trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol
of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25
°C.A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin
Unit = 1800 KIU.
Recombinant aprotinin should be stored under -20°C or below in
sealed container.It is stable within 24 months.