Chymotrypsin Enzyme with Sequencing Grade impact for Protein
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human
chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl
side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage
may also be observed, but at a lower rate, at Leu and Met.
Chymotrypsin activity is optimal in pH 7.0–9.0.
The sequencing grade human chymotrypsin can be used alone or in
combination with other proteases
to produce protein digests for peptide mapping applications or
protein identification by peptide mass fingerprinting or MS/MS
spectral matching. It is suitable for digestion reactions
in-solution or in-gel.
≥ 98 % by HPLC.
≥ 1500 units/mg pro
1) Protein digests for peptide mapping applications or protein
identification by peptide mass
fingerprinting or MS/MS spectral matching. It is suitable for
digestion reactions in-solution or in-gel.
2) This sequencing grade enzyme can be used alone or in combination
with other proteases to produce.
For peptide digestion, use a ratio (w/w) of approximately
1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40
mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.
(1)Animal origin free: Recombinant human chymotrypsin is no
exogenous virus contamination,and any animal origin material is not
used in the production process.
(2)Stable quality: Mass production can ensure stable and continuous
batch production.It is no difference between the batch and the
product quality is stable.
(3)High purity: Higher specific activity.Host protein residues is
less than the limits of biological products.
(4)Lyophilized powder: The product is lyophilized powder and is
easy to store and transport.
(5)Compliance with regulatory requirements: Production equipment
and production environment comply with relevant regulatory
requirements, and the production process is in full compliance with
NSF ISO 9001: 2008 quality system and GMP guidelines.
(6)Complete quality documents: we can provide relevant regular
support files in according to customers’ requirement.
Used alone, or with other proteases to interact with digestive
proteins, or other special uses such as organic synthesis.
Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃
in sealed container. It is stable within 24 months.After dissolved,
it should be stored under -20℃. It is stable within 24 months and
above 90% activity remained after 10 times repeated freezing and