Recombinant human Chymotrypsin Enzyme for Enzymatic Hydrolysis of
Source: human chymotrypsin, expressed in E. Coli.
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human
chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl
side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage
may also be observed, but at a lower rate, at Leu and Met.
Chymotrypsin activity is optimal in pH 7.0–9.0.
Animal origin free:Recombinant human chymotrypsin eliminates the
risk of virus presence, and other potential adventitious agents
found in animal-derived chymotrypsin.
High purity:Recombinant Chymotrypsin provides increased specificity
and eliminates contaminated other proteases activities found in
enzymes purified from pancreas.
Stable:A sterile recombinant human chymotrypsin lyophilized
decreases the contamination risks and chance of activity loss
during transport and storage.
Hydrolysis of proteins by chymotrypsin alone or in combination with
other proteases. Suitable for peptide mapping, fingerprinting, and
White lyophilized r
> 95% by HPLC
Unit Definition:One unit will hydrolyze 1.0 μmole of BTEE per min
at pH 7.8 at 25 °C.
Prepare 1-10mg/ml with 1ml 50mM HAc, used within 2 days, or stored
below -20℃ after repacked
Recombinant carboxypeptidase B
Sequencing grade chymotrypsin