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Lyophilized Powder Chymotrypsin Enzyme Recombinant Human Chymotrypsin for Mass Spectrometry

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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Tel: 86-21-54336592
Contact Person:
Mr zhao

Lyophilized Powder Chymotrypsin Enzyme Recombinant Human Chymotrypsin for Mass Spectrometry

Brand Name : YaxinBio
Model Number : RCT10
Certification : NSF ISO 9001
Place of Origin : China
MOQ : 10mg
Payment Terms : T/T
Packaging Details : ice packaging
Price : 89$/10mg
Supply Ability : 1000g per week
Product : Recombinant Chymotrypsin
Source : E. Coli
RELATED PRODUCT  : Recombinant Trypsin
Physical form : White lyophilized powder
Specific activity : ≥1000 unit/mg pro
Manufacturer : YaxinBio
usage : Mass Spectrometry
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Lyophilized Powder Chymotrypsin Enzyme Recombinant Human Chymotrypsin for Mass Spectrometry


CAS: 9004-07-3
EC: 3.4.21.1


Description


Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.


MAIN FEATURES


SourceRecombinant E. coli
AppearanceWhite or off white powder
Specific activity≥1000 USP units/mg pro.
SDS-PAGESingle major band
SDS-PAGE (MW)24.0±2.4 kDa

UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.


RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.


STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.


Advantages


Animal origin free:Recombinant human chymotrypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

High purity:Recombinant Chymotrypsin provides increased specificity and eliminates contaminated other proteases activities found in enzymes purified from pancreas.

Stable:A sterile recombinant human chymotrypsin lyophilized decreases the contamination risks and chance of activity loss during transport and storage.


Email:Zhaozhi@yaxinbio.com


Product Tags:

human chymotrypsin

      

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Lyophilized Powder Chymotrypsin Enzyme Recombinant Human Chymotrypsin for Mass Spectrometry Images

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