Lyophilized Powder Chymotrypsin Enzyme Recombinant Human
Chymotrypsin for Mass Spectrometry
Recombinant human chymotrypsin
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human
chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl
side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage
may also be observed, but at a lower rate, at Leu and Met.
Chymotrypsin activity is optimal in pH 7.0–9.0.
|Source||Recombinant E. coli|
|Appearance||White or off white powder|
|Specific activity||≥1000 USP units/mg pro.|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||24.0±2.4 kDa|
UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine
ethyl ester(ATEE) per min at pH 7.0 at 25 °C.
Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The
ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human chymotrypsin lyophilized
should be stored under 2-8℃ in sealed container.It is stable within
24 months.After dissolved wth 1mM HCl , it should be stored under -20℃. Avoid repeated
freezing and thawing.
Stability of transport: The product is stable by bue ice insulation transport.
Animal origin free:Recombinant human chymotrypsin eliminates the
risk of virus presence, and other potential adventitious agents
found in animal-derived chymotrypsin.
High purity:Recombinant Chymotrypsin provides increased specificity
and eliminates contaminated other proteases activities found in
enzymes purified from pancreas.
Stable:A sterile recombinant human chymotrypsin lyophilized
decreases the contamination risks and chance of activity loss
during transport and storage.